Our PC2 mammalian cell laboratory enables researchers to establish, store, culture, view and work with animal cells; from human Jurkat to HeLa, to cancerous green monkey kidney, to rat epithelia.
This ISO 7 Class 10,000 laboratory is certified by the Office of the Gene Technology Regulator. Users can safely work with a broad variety of mammalian cell lines without concern for contamination or viability to serve as an ex vivo test bed for many of the technologies developed in other parts of the facility.
Several internationally competitive research activities can be pursued in this laboratory. An example is the improvement of tissue constructs through three-dimensional culturing, vascularisation, and cellular differentiation to provide useful tissue and organ replacement using micro to nano fluidics for cellular manipulation. Another example is the engineering of specific proteins and peptides, like listerolysin and perforin, into structured nanoparticles containing silencing or messenger RNA for the treatment of cancer, autoimmune disease, and diabetes.
- Biological atomic force microscope (BFM)
- Flow cytometer cell sorter (FAC)
- Stochastic optical reconstruction microscope and confocal system (NST)
Biological atomic force microscope (BFM)
The Asylum MFP-3D Biological Atomic Force Microscope performs imaging and characterisation of materials in liquid and air. It can be used to image the topography of soft biological materials in their native environments, and probe mechanical properties of cells and extracellular matrices.
Flow cytometer cell sorter (FAC)
The BD Biosciences FACSJazz cell sorter system performs detection, analysis, and sorting of cells excited by two lasers (488nm and 640nm). The instrument is capable of two way sorting into 5ml polypropylene tubes, 15 ml conical tubes and well plates (6, 24, 48, 96 and 384).
Stochastic optical reconstruction microscope and confocal system (NST)
The Nikon N-STORM coupled with Nikon’s inverted microscope allows imaging at tenfold the resolution of conventional optical microscopes (approximately 20 nm in xy). The system can be also used for epifluorescence confocal microscopy.
The laboratory also contains a broad range of tools to assist researchers in using the equipment. Full details are provided in the training modules and manuals for the laboratory.