Professor Andrew Smith is Dean, School of Applied Sciences at RMIT University.
Areas of research interest
Structure/function relationships and mechanisms of metalloenzymes with particular emphasis on haem systems and peroxidases, particularly those that play a role in the degradation of lignin, the recalcitrant biopolymer that gives wood its strength. Degradation of lignin is known to be rate limiting in the carbon cycle and clean technologies that would help us unlock lignin and hence cellulose as renewable sources of chemicals and fuels may be of great importance in the future. Professor Smith’s research group is exploring the relatonships between globins, peroxidases and cytochromes P450 using horseradish peroxidase and lignin peroxidase as our main model systems. We are attempting to understand the structural determinants of activity and specificity with a view to creating new catalytic activities, such as the ability to fragment lignin (WO/2006/114616) or selectively oxygenate substrates (WO/2007/020428). The research is multidisciplinary in nature and at the interface between Chemistry and Biology. Recent highlights have included the structure of HRP in all five oxidation states (in collaboration with others) published in Nature (2002) and the role of a redox active Trp residue in the catalytic cycle of an engineered lignin peroxidase published in PNAS (2009).
External and visiting appointments
- Visiting Prof, Department of Chemistry and Biochemistry, School of Life Sciences, University of Sussex UK.
Affiliations and professional memberships
- Inorganic Biochemistry Discussion Group, Biochemical Society
- BSc and AUS (University of Surrey, UK)
- PhD (University of Southampton, UK)
- Golneshin, A.,Adetutu, E.,Ball, A.,May, B.,Van, T.,Smith, A. (2015). Complete genome sequence of lactobacillus plantarum strain B21, a bacteriocin-producing strain isolated from Vietnamese fermented sausage Nem Chua In: Genome Announcements, 3, 1 - 2
- Taha Elfouly, M.,Kadali, K.,Alhothaly, K.,Smith, A.,Ball, A.,Adetutu, E. (2015). An effective microplate method (Biolog MT2) for screening native lignocellulosic-straw-degrading bacteria In: Annals of Microbiology, 65, 2053 - 2064
- Taha, M.,Adetutu, E.,Shahsavari, E.,Smith, A.,Ball, A. (2014). Azo and anthraquinone dye mixture decolourization at elevated temperature and concentration by a newly isolated thermophilic fungus, Thermomucor indicae-seudaticae In: Journal of Environmental Chemical Engineering, 2, 415 - 423
- Zhou, X.,Entwistle, A.,Zhang, H.,Jackson, A.,Mason, T.,Shimanovich, U.,Knowles, T.,Smith, A.,Sawyer, E.,Perrett, S. (2014). Self-assembly of amyloid fibrils that display active enzymes In: ChemCatChem, 6, 1961 - 1968
- Giordano, A.,Smith, A.,Mouradov, A. (2013). Metabolic reprogramming of lignin biosynthesis in crops In: Signpost Open Access Journal of NanoPhotoBioSciences, 1, 93 - 117
- Smith, A.,Doyle, W.,Dorlet, P.,Ivancich, A. (2009). Spectroscopic evidence for an engineered catalytically-active trp radical that creates the unique reactivity of lignin peroxidase In: Proceedings of the National Academy of Sciences, 106, 1684 - 1689
- Wiseman, B.,Smith, A.,Colin, J.,Ivancich, A.,Loewen, P. (2009). Mechanistic insight into the initiation step of the reaction of Burkholderia pseudomallei catalase-peroxidase with peroxyacteic acid In: Journal of Biological Inorganic Chemistry, 14, 801 - 811
- Ruiz-Duenas, F.,Morales, M.,Mate, M.,Romero, A.,Martinez-Canet, M.,Smith, A.,Martinez, A. (2008). Site-directed mutagenesis of the catalytic tryptophan environment in Pleurotus eryngii versatile peroxidase In: Biochemistry, 47, 1685 - 1695
- Howes, B.,Brissett, N.,Doyle, W.,Smith, A.,Smulevich, G. (2005). Spectroscopic and kinetic propeties of the horseradish peroxidase mutant T171S: Evidence for selective effects on the reduced state of the enzyme In: The FEBS Journal, 272, 5514 - 5521
- O'Brien, A.,Smith, A.,O'Faigain, C. (2003). Effects of phthalic anhydride modification on horseradish peroxidase stability and activity In: The Journal of Biotechnology and Bioengineering, 81, 233 - 240
1 PhD Current Supervisions2 PhD Completions