Course Title: Perform aseptic techniques
Part B: Course Detail
Teaching Period: Term1 2018
Course Code: BIOL5227C
Course Title: Perform aseptic techniques
School: 174T School of VE Engineering, Health & Science
Campus: City Campus
Program: C5363 - Diploma of Laboratory Technology (Biotechnology)
Course Contact: Namrita Kaul
Course Contact Phone: +61 3 9925 4837
Course Contact Email: namrita.kaul@rmit.edu.au
Name and Contact Details of All Other Relevant Staff
Andrea McAdam
andrea.mcadam@rmit.edu.au
51.7.05
Nominal Hours: 40
Regardless of the mode of delivery, represent a guide to the relative teaching time and student effort required to successfully achieve a particular competency/module. This may include not only scheduled classes or workplace visits but also the amount of effort required to undertake, evaluate and complete all assessment requirements, including any non-classroom activities.
Pre-requisites and Co-requisites
None
Course Description
This unit of competency covers the ability to perform aseptic techniques to maintain the integrity of both the sample source and the sample. It applies to sampling techniques in tissue culture and to generic microbiological procedures.
National Codes, Titles, Elements and Performance Criteria
National Element Code & Title: |
MSL973004 Perform aseptic techniques |
Element: |
1 Prepare for aseptic sampling or transfer |
Performance Criteria: |
1.1 Ensure that any sampling procedure conforms with the requirements of the sampling plan |
Element: |
2 Transfer materials aseptically |
Performance Criteria: |
2.1 Protect the integrity of the sample source by sterilising the sampling site and the mouth of transport or culture vessel |
Element: |
3 Maintain work area and equipment to prevent cross-infection and contamination |
Performance Criteria: |
3.1 Place disposable and reusable items into relevant receptacles |
Learning Outcomes
Details of Learning Activities
Practical techniques performed in laboratory
Online information in Blackboard
Additional activities for out-of-class individual study and investigation
Teaching Schedule
Week 10 Mon 16 April
Theory
A1-1 Introduction to aseptic technique
A1-2 Patient specimens
Prac
Introduction to aseptic technique
Hand washing / Environmental swab / Lawn culture
Week 11 Mon 23 April
Theory
A1-1 Introduction to aseptic technique
A1-2 Patient specimens
Prac
Make media, Autoclave
Streak for single colonies
Review plates from Week 10 A1
Week 12 Mon 30 April
Theory
A3-1 Media
A3-2 Disinfection
A3-3 Cross-contamination
Prac
Effectiveness of disinfection
Plating from swabs on bench
Review plates from Week 9 A2
Week 13 Mon 7 May
Theory
A4-1 Reading plates
A4-2 Gram staining
Prac
Plating from swabs
Gram stains / oil immersion
Review plates from Week 12 A3
Week 14 Mon 14 May
Theory
A5-1 Antibiotic sensitivities
A5-2 Biosafety cabinets
Prac
Antibiotic sensitivity
Plating in Biosafety cabinet
Review plates from Week 13 A4
Week 15 Mon 21 May
Theory
A6-1 Revision
Prac
Review plates from Week 14 A5
Practical Assessment
Week 16
END SEMESTER EXAMS – Written assessment
Learning Resources
Prescribed Texts
References
Other Resources
Overview of Assessment
practical skills assessment
laboratory logbook
online quizzes
glossary
Assessment Tasks
Logbook record of practical classes and post-class exercises (S/NYS).
At least five out of six practical classes must be completed.
Practical assessment: streak an agar plate aseptically for single colonies ( S/NYS)
Written test (S/NYS) 75% pass considered satisfactory.
All assessment types must be completed to a satisfactory standard to pass this course.
Assessment Matrix
Other Information
This course is an ungraded competency. It is a Pass only outcome – no higher grading. Outcome will be one of the following:
CA- Competency Achieved
NYC- Not Yet Competent
DNS- Did Not Submit for assessment
- All assessment types must be passed (exams, prac, and assignments etc.).
You can’t make up marks from one type of assessment to another (e.g. pass the tests but fail the prac component). - Attendance at practical classes is compulsory. A minimum of 80% attendance is required to pass this course. That is, 5 out of 6 practical classes. If you are sick you should submit a medical certificate to 6th floor office addressed to the Program Coordinator
- Late work that is submitted without an application for an extension will not be corrected.
- APPLICATION FOR EXTENSION OF TIME FOR SUBMISSION OF ASSESSABLE WORK- A student may apply for an extension of up to 7 days from the original due date. They must lodge the application form (available online http://www1.rmit.edu.au/students/assessment/extension) at least 24 hours before the due date. The application form should be emailed to the program Coordinator (namrita.kaul@rmit.edu.au).
- Students requiring extensions longer than 7 days must apply for Special Consideration (see the 'Help me' link in Blackboard via myRMIT studies or http://www.rmit.edu.au/students/specialconsideration). For missed assessments such as exams, you (and if you are sick, your doctor) must complete a Special Consideration form and submit it online with supporting evidence (eg medical certificate) before, or within 48hours of the scheduled assessment.
Course Overview: Access Course Overview